Figure 5—figure supplement 5. Structural analysis of H3K64 interactions.(A) Distances in Å between DNA backbone phosphate oxygen atoms and H3K64 main-chain amide (red text) or terminal nitrogen atoms (black text) in the high-resolution (1.9 Å) crystal structure of the nucleosome (PDB ID 1KX5). The H3K64 main-chain amide directly interacts with the DNA phosphate backbone, this places the H3K64 side-chain within a range to form direct interactions with the DNA. The measured distances are shown as yellow-dashed lines. (B) Two alternate side-chain rotamer conformations of H3K64 (light blue) are shown that demonstrate that the H3K64 side-chain has the potential to form direct ionic interactions with the DNA phosphate backbone. (C) The side-chain of H3K64 is part of an extensive water-mediated hydrogen bonding network between histones and DNA in the high-resolution crystal structure of the nucleosome (PDB ID 1KX5). Water atoms are shown as red spheres and hydrogen bonds as dashed black lines. Each of the displayed hydrogen bonds can be traced back to the water molecule bound to H3K64.DOI: http://dx.doi.org/10.7554/eLife.01632.020
Image published in: Di Cerbo V et al. (2014)
Copyright © 2014, Di Cerbo et al. Creative Commons Attribution license
Permanent Image Page
Printer Friendly View
XB-IMG-168822