Figure 1 CS expression in the Xenopus embryonic optic pathway. Confocal micrographs of transverse sections of stage 37/38 embryos immunostained for endogenous CSs. (A) CS immunostaining, as visualized by the CS-56 antibody, is localized in the basal lamina surrounding the diencephalon and to a lesser extent in the neuropil (Np) and the neuroepithelium (Ne). There is also abundant CS expression in the mesenchyme outside the nervous system. Arrowheads represent the area enlarged in panel E. (B–D) Specific expression of the CS subtypes. (B) Chondroitin is expressed at higher levels in the neuropil than in the neuroepithelium. (C) Chondroitin-4-sulfate is expressed in both the neuropil and neuroepithelium. (D) Chondroitin-6-sulfate is expressed primarily in the neuroepithelium and at a lower degree in the neuropil. (E) A double-labeled section showing HRP-filled RGC axons (red) growing in the CS-rich region (green) of the diencephalic neuropil. Dorsal is up. Scale bar is 50 m in (A)–(D) and 25 m in (E).
Image published in: Walz A et al. (2002)
Copyright © 2002. Image reproduced with permission of the Publisher.
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