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Figure 1. ERK3 expression in Xenopus laevis embryos. A, Real-time quantitative RT-PCR analysis. The expression levels of ERK3 were normalized to those of odc in two independent experiments (#1 and #2). The normalized ERK3 expression level at stage 1 was defined as 1.0 in each experiment. B, Whole-mount in situ hybridization analysis of ERK3 expression. ec, ectoderm; nc, neural crest; s, somite; nt, neural tube; ep, epidermis; pn, pronephros; b, brain; e, eye. Scale bars, 400 m. Anterior is to the left (stage 19-33/34). Dorsal is up for the lateral views (stage 19-33/34). Shown are representative images of 6-10 embryos from one experiment using the ERK3A probe. Essentially the same results were obtained for 6-9 embryos from another experiment using the ERK3B probe.

Image published in: Takahashi C et al. (2018)

Copyright © 2018. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
mapk6.Lerk3, p97mapk, prkm6X. laevisThroughout NF stage 12ectoderm
animal cap
mapk6.Lerk3, p97mapk, prkm6X. laevisThroughout NF stage 19neural tube
neural crest
somite
epidermis
mapk6.Lerk3, p97mapk, prkm6X. laevisSometime during NF stage 25 to NF stage 33 and 34brain
eye
neural tube
pronephric kidney
pronephric mesenchyme
neural crest
epidermis
somite
mapk6.Lerk3, p97mapk, prkm6X. laevisThroughout NF stage 9animal hemisphere

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