Fig. 8. Rescue of Dishevelled stability by dn-caveolin and proteosome inhibitor. Western blot analysis of XDsh-Myc at the shield stage of zebrafish embryos. (A) Absence of effect of dscr5 knockdown on the stability of Wnt11-Myc, XFz7-Myc and endogenous β-Catenin. (B) Dose-dependent effect of dscr5 knockdown on XDshMyc stability. (C) XDsh-Myc degradation in embryos injected with gly4ÎC RNA or knyMO. (D) Coexpression of dn-caveolin rescues XDshMyc stability in dscr5 morphants. (E) MG132, but not NH4Cl, maintains XDsh-Myc stability in dscr5 knockdown cells. Endogenous JNK was used as a loading control.
Image published in: Shao M et al. (2009)
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