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Larger Image Fig. 4. The expression of gtpbp10 in late stages of development shown by whole-mount in situ hybridization (WISH). gtpbp10 antisense riboprobe was used for in situ hybridization on A) Early tail bud stage embryo (stage 28) and B) Late tail-bud stage (stage 36). Aâ² and Bâ² are transverse sections of the embryos shown in A and B at the level of the dotted white lines. Structures labeled as follows: notochord (n), neural tube (nt), forebrain (fb), hindbrain (hb), pronephros (pn), branchial arches (ba). * denotes the glomerulus and proximal tubules, while âtâ indicates the distal tubules. C) gtpbp10 sense probe shows background staining in the anterior branchial arches, otic vesicle, eye, and fore-, mid-, and hindbrain. Staining was also observed in somitic tissue (so) along the anterior-posterior axis. Image published in: Jerry R et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V.
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