Larger Image Figure 1: Digitonin-induced permeabilization of fin cells in suspension. Mesenchymal fin cells were tested for their ability to respond to various digitonin treatments. (A) Permeabilization rates and cell recovery rates after 2 min incubation. (B) Response over time of digitonin-induced permeabilization (2 to 24 min). Cells were incubated at digitonin concentrations ranging from 5 to 30 µg/mL and labelled with the non membrane permeant propidium iodide (PI). Cell permeabilization rates are expressed as a percentage of PI-positive cells to the total cell number in the sample. Cell recovery rates are expressed as a percentage of the cell number after digitonin treatment to the initial cell number before treatment. Bars represent means ± SD (n = 3 to 13 independent fin cell suspension). In (A), different letters indicate significant differences (p < 0.01) for a given concentration. Image published in: Chênais N et al. (2019) © The Author(s) 2019. Creative Commons Attribution license Permanent Image Page Printer Friendly View XB-IMG-181396