Figure S3. Eliminating putative TMPRSS2 cleavage sites distal to the γ-inhibitory tract prevents the appearance of fully cleaved γ-ENaC in whole-cell lysates. All western blots obtained in similar experiments as shown in Fig. 8C are depicted. Parts of blots shown in Fig. 8C are framed by dotted rectangles. Whole-cell expression of γ-ENaC was detected using an antibody against a C-terminal γENaC epitope in oocytes expressing wild-type (αβγ) or mutant ENaC (αβγRKRK178AAAA, αβγK168A;K170A;R172A;K189A or αβγRKRK178AAAA;K168A;K170A;R172A;K189A) either without (−) or with (+) TMPRSS2 coexpression. Non-injected oocytes served as control (n.i.). Expression of γ-ENaC was analysed for αβγRKRK178AAAA- (A) and αβγK168A;K170A;R172A;K189A- (B) in four, for αβγRKRK178AAAA;K168A;K170A;R172A;K189A- (C) in six, and for αβγ-ENaC (A-C) in nine different repeats indicated by circles of different colours. Positions of uncleaved (~87 kDa), partially cleaved (~76 kDa) and fully cleaved γ-ENaC (~67 kDa) are indicated by open, light grey and dark grey filled arrowheads, respectively.
Image published in: Sure F et al. (2022)
Copyright © 2022. Image reproduced with permission of the Publisher.
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