Fig. 3. Gauging coassembly of hBest1 with other CACC subunits by shifts in single-molecule subunit counts of mEGFP–hBest1. (A–E) Frequency distributions of single-molecule–photobleaching counts of mEGFP–hBest1 coexpressed with untagged hBest1 (number of optical patches n = 6) (A), hBest2 (n = 7) (B), hBest3 (n = 5) (C), hBest4 (n = 4) (D), or mTMEM16A (n = 5) (E), compared with the binomial distribution of tetramer with the probability that mEGFP is fluorescent = 0.8 (red dashed lines). (F) Fraction of spots with four bleaching steps when mEGFP–hBest1 is expressed alone (mEGFP–B1) or together with untagged hBestx (mEGFP–hBest1 + Bx) or untagged mTMEM16A (mEGFP–hBest1 + mTMEM16A).
Image published in: Bharill S et al. (2014)
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