Figure 3. PCNA Modification on Lysine 164 and Pol κ UBZ Domains Are Required for RIR (A) Quantification of ICL repair in mock- and Rad1-depleted HSS. See also Figure S3. (B) Quantification of ICL repair in mock-depleted HSS, PCNA-depleted HSS, and PCNA-depleted HSS supplemented with PCNAWT or PCNAK164R. (C) Control or ICL plasmids were incubated in extract for 40 min and immobilized using biotinylated LacR protein. DNA-bound proteins were analyzed by western blotting. (D) Quantification of ICL repair in mock-, Pol κ-, or Pol κ-depleted HSS supplemented with Pol κWT, Pol κD634A,D789A, or Pol κI857A,F860A,F861A, as indicated. Results represent the mean and SEM from at least three independent experiments.
Image published in: Williams HL et al. (2012)
Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V.
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