Fig. 8. rfng overexpression caused ectopic proximal pronephrogenesis. (A-I) X. laevis embryos were injected with rfng mRNA as previously described. rfng overexpression causes ectopic Lim-1 (A) and hrt1 expression (B) at stage 28. At stage 41, 3G8 and 4A6 immunostaining is ectopic; frequently the entire proximal pronephros is duplicated in the distal region (white arrow, C). Similarly, Na+K+ATPase (D), slc5a2 (E) and nephrin (F) expression is also ectopic. GATA-3, which is expressed solely in the distal tubule, is completely absent (G). Co-injection of rfng mRNA with the wnt4 MO abrogates these effects; nephrin (H) and slc5a2 (I) expression is reduced in these embryos. Asterisk denotes injected side.
Image published in: Naylor RW and Jones EA (2009)
Copyright © 2009. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| lhx1.L | lim-1, Lim1, lim1, Xlim-1, Xlim1 | X. laevis | Throughout NF stage 26 | pronephric mesenchyme |
| hey1.S | bc8, chf2, herp2, hesr1, hrt-1, hrt1, oaf1, XHRT1 | X. laevis | Throughout NF stage 28 | pronephric mesenchyme glomeral mesenchyme |
| atp1b1.S | atp1b1a, Beta1-Na/K-ATPase, Na+-K+-ATPase, β1-NaK-ATPase | X. laevis | Throughout NF stage 28 | early proximal tubule late proximal tubule early distal tubule late distal tubule |
| slc5a2.L | X. laevis | Throughout NF stage 28 | early proximal tubule late proximal tubule | |
| nphs1.S | nephrin | X. laevis | Throughout NF stage 28 | glomeral mesenchyme |
| gata3.S | GATA-3, XGATA-3, XGATA3 | X. laevis | Throughout NF stage 28 | early distal tubule late distal tubule |
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