Fig. 5. Triple PG1 knockdown severely affects hindbrain patterning. Morpholinos for each of the Hox PG1 genes (A1/B1/D1MO) were co-injected into the lhs of the embryo (B,D,F,H,J). Non-injected controls are also shown (A,C,E,G,I). The hindbrain region is shown, with anterior to the top. Expression of the neural marker Nrp1 (A,B: 100%, n=11), the anterior marker Otx2 (C,D: 100%, n=10), Gbx2, which is expressed in r1 (E,F: 77%, n=13) and Krox20 (G,H: no stripes 41%; one faint stripe 35%, n=71), was analysed. Dotted line indicates the midline. In later embryos (st. 46), the neural antibody 2-G9 was used to show the morphology of the hindbrain [NIC, I; A1/B1/D1MO, injected on the left-hand side (lhs) *, J: 100%, n=5]. Rhombomere boundaries are marked.
Image published in: McNulty CL et al. (2005)
Copyright © 2005. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| nrp1.L | A5 antigen, LOC108718763, neuropilin, neuropilin-1, np-1, npn-1, nrp, nrp-1, pNPG152 | X. laevis | Throughout NF stage 22 | neural plate midbrain hindbrain neural tube posterior neural tube anterior neural tube forebrain telencephalon diencephalon spinal cord eye otic placode optic vesicle |
| otx2.S | otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 | X. laevis | Throughout NF stage 22 | anterior eye optic vesicle forebrain cement gland primordium |
| gbx2.S | gbx2.1, XGBX-2 | X. laevis | Throughout NF stage 22 | hindbrain midbrain-hindbrain boundary cranial neural crest rhombomere otic placode |
| myod1.S | mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD | X. laevis | Throughout NF stage 22 | somite muscle |
| egr2.L | EGR-2, Krox-20, krox20, XKr20, XKrox-20 | X. laevis | Throughout NF stage 24 | hindbrain cranial neural crest rhombomere R3 rhombomere R5 anterior branchial crest |
| lsamp.L | 2G9, iglon3, lamp, LOC108709279 | X. laevis | Throughout NF stage 46 | midbrain hindbrain neural tube rhombomere rhombomere R2 rhombomere R3 rhombomere R4 rhombomere R5 rhombomere R6 brain forebrain |
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