Fig. 2. WISH analysis of Wnt4 mRNA expression in the VBI and DLP in Xenopus embryos. (AâD) Wnt4 transcripts are observed in the neural plate and in the VBI and DLP mesoderm in stage-21 embryos. (B) Transverse section at site indicated by dashed line in A. (C) Closer view of dorsolateral and ventral regions (D). Wnt4 signal is clearly excluded from overlying ectoderm (C and D). Wnt4 transcripts in DLP are sustained in embryos at stage 25 (E) and restricted to pronephric region in embryos at stage 28 (F) and stage 34 (G). Wnt4 is clearly expressed in VBI (EâH) and specifically in the mesodermal layers (arrowhead in H), but not in overlying ectoderm (arrow in H). Wnt4 is also expressed in the brain, branchial arches, and lateral plate mesoderm (arrowhead in C). dlp, dorsal lateral plate, dlpm, dorsal lateral plate mesoderm; ecto, ectoderm; endo, endoderm; hb, hindbrain; hend, hepatic endoderm; mb, midbrain; mhb, midbrainâhindbrain boundary; meso, mesoderm; np, neural plate; nt, neural tube; pt, pronephric tubules; vbi, ventral blood island.
Image published in: Tran HT et al. (2010)
Copyright © 2010. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| wnt4.L | wnt-4, Xwnt-4, Xwnt4 | X. laevis | Throughout NF stage 21 | neural tube mesoderm ventral blood island lateral plate mesoderm anterior dorsal lateral plate region |
| wnt4.L | wnt-4, Xwnt-4, Xwnt4 | X. laevis | Throughout NF stage 25 | brain mesoderm ventral blood island lateral plate mesoderm anterior dorsal lateral plate region neural tube hematological system vascular plexus pharyngeal arch head region |
| wnt4.L | wnt-4, Xwnt-4, Xwnt4 | X. laevis | Throughout NF stage 28 | forebrain pharyngeal arch brain hindbrain mesoderm ventral blood island anterior dorsal lateral plate region neural tube midbrain pronephric tubule liver primordium |
| wnt4.L | wnt-4, Xwnt-4, Xwnt4 | X. laevis | Throughout NF stage 33 and 34 | brain hindbrain mesoderm ventral blood island lateral plate mesoderm anterior dorsal lateral plate region neural tube midbrain pronephric tubule hematological system pharyngeal arch midbrain-hindbrain boundary |
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