Fig. 7. Cooperative effects and physical interactions within the Gli superfamily. (A) (Top left) Human GLI1 is nuclear in transfected COS cells, here detected with specific polyclonal anti-human GLI antibodies (Lee et al., 1997). (Top right) Myc-tagged Gli2 is cytoplasmic. (Bottom left) Co-expression of GLI1 and Gli2 results in nuclear localization of Gli2 (bottom right). (B) (Top panels) Expression of Myc-tagged frog Gli1, Myc-tagged-Gli2, Myc-tagged GLI3 and FLAG-tagged Zic2 in COS cells. (Bottom panels) Co-expression with Zic2 forces the Gli1, Gli2 and GLI3 cytoplasmic proteins into the nucleus. DAPI staining highlights nuclei (blue). The color of the font indicates the protein visualized by immunostaining. (C,D) Quantification of the subcellular compartmentalization of Gli proteins when transfected alone versus co-transfection with human GLI1 (C), and when transfected alone versus co-transfection with Zic2 (D). (C) Only nuclear values are given for co-transfection of Gli2 and GLI1 and for GLI3 and GLI1. (E-H) (Top panels) Immunoprecipitation of Flag-tagged proteins with anti-FLAG Ab as labeled, followed by western blotting with anti-Myc Ab. Arrowheads indicate the immunoprecipitated species. (Bottom panels) Corresponding western analysis of total Myc-protein input from transfected COS cells as labeled. The horizontal alignment of bottom panels corresponds to the molecular weight markers on the left.
Image published in: Nguyen V et al. (2005)
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