Larger Image Figure 4. Expression of Gremlin in regenerating WT and N1 limbs and during development Expression of Gremlin in regenerating WT and N1 limbs and during development. Gene expression in regenerating wild type (WT) and N1 limbs and embryo tissue. (A-J) In situ hybridisation showing Gremlin expression in the regeneration bud. (L-O) Unoperated limb buds illustrating Gremlin expression during limb development. (K) In situ hybridisation showing Gremlin expression in a stage 32 embryo, the expression pattern is consistent with previously published Gremlin embryo expression [30]. White arrowheads indicate approximate amputation plane, scale bar in A applies to panels A-J and scale bar in O to pan- els L-O. In limb panels (A-J, L-O) posterior is uppermost, and distal to the left, dr = days of regeneration. In K, anterior is to the left and dorsal uppermost. White asterisks mark areas of Gremlin expression that are developmental and unrelated to regeneration. Image published in: Pearl EJ et al. (2008) Copyright © 2008 Pearl et al; licensee BioMed Central Ltd. Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue grem1.L Cktsf1b1, cysteine knot superfamily 1, gremlin X. laevis Throughout NF stage 32 pronephric duct pronephric kidney intersomitic region pharyngeal arch tail fin tail tip mandibular arch hyoid arch branchial arch cephalic nerve ventral fin margin grem1.L Cktsf1b1, cysteine knot superfamily 1, gremlin X. laevis Throughout NF stage 50 to NF stage 53 limb bud distal mesenchyme hindlimb bud grem1.L Cktsf1b1, cysteine knot superfamily 1, gremlin X. laevis Throughout NF stage 54 blastema distal mesenchyme mesenchyme Image source: Published Experiment + Assay Source Phenotypes and Disease Xla.Tg(hsp70:nog,cryga:GFP) + hindlimb bud amputation + HS + NF53 Fig. 4 I Expression Phenotype decreased amount grem1.L expression in apical mesenchyme Xla.Tg(hsp70:nog,cryga:GFP) + hindlimb bud amputation + HS + NF53 Fig. 4 J Expression Phenotype decreased amount grem1.L expression in apical mesenchyme Permanent Image Page Printer Friendly View XB-IMG-74568