Fig. S2. Overexpression of H3.3 leads to early embryonic lethality. (A) Embryos at the two- to four-cell stages were injected with 1 or 2 ng of mRNA encoding HA-tagged H3.1 or H3.3. Injected embryos were either allowed to develop to late tailbud stage, or fixed at stage 10.5 and subjected to whole-mount in situ hybridization analysis of Xbra expression. (B) Percentage of injected embryos that developed normally to late gastrula stage 12.5. (C) Synthesis of exogenous H3.1- HA or H3.3-HA proteins in injected embryos detected by immunoblotting for the HA epitope.
Image published in: Lim CY et al. (2013)
Copyright © 2013. Image reproduced with permission of the Publisher.
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