Larger Image Fig. S8. BMP signaling that function in a time period after respiratory specification is required for trachea formation and lung bud development. (A,B) In situ hybridization of nkx2.1 expression in embryos treated from stages 35-42 with DMSO (vehicle) or 10 µM LDN193189 to inhibit BMP receptor type I signalling. Red arrows show absent trachea and single dismorphic lung bud. Brain, br; trachea, tr; lung bud, lb. (C,D) Immunofluorescence analysis of Nkx2.1 (green) and Sox2 (red) in serial foregut sections (1 is the anterior-most section, 4 is the posterior-most) reveal loss of trachea, expansion of esophagus and lung bud hypoplasia in LDN-treated embryos. Image published in: Rankin SA et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher. Experiment + Assay Source Phenotypes and Disease Xtr Wt + LDN-193189 + NF42 (in situ hybridization) fig.S8.b Anatomical Phenotype abnormal agenesis of trachea decreased size of the lung bud Expression Phenotype decreased amount nkx2-1 expression in lung bud decreased amount nkx2-1 expression in trachea Xtr Wt + LDN-193189 + NF42 (immunohistochemistry) fig.S8.d1,d2,d3 Anatomical Phenotype abnormal agenesis of trachea abnormal esophagus morphology Expression Phenotype decreased amount nkx2-1 expression in trachea Xtr Wt + LDN-193189 + NF42 (immunohistochemistry) fig.S8.d3,d4 Anatomical Phenotype decreased size of the lung bud Expression Phenotype decreased amount nkx2-1 expression in lung bud Permanent Image Page Printer Friendly View XB-IMG-80978