Fig. 9. Repression of xBMP-3b and cerberus leads to impaired gastrulation movements. Lineage of cells targeted by MOs and expression of marker genes were monitored at gastrula, neurula and tadpole stages. (Left) Embryos injected with MOs for both xBMP-3b and cerberus. (Right) Control embryos injected with globin MO. Light blue staining represents cells targeted by MOs. Brown staining represents expression of markers as indicated on right corner. Dorsal side faces top in panels of gastrula and tadpole, and anterior side faces top in panels of neurula. Injection of MOs for xBMP-3b and cerberus did not affect dorsal lip formation and expression of organizer genes at beginning of gastrulation, but led to delayed blastopore closure and downregulation of Otx2 expression by neurula stage. Consequently, suppressing xBMP-3b and cerberus functions elevated expression of a ventral–posterior marker, Xcad1.
Image published in: Hino J et al. (2003)
Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| otx2.S | otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 | X. laevis | Throughout NF stage 11 | dorsal marginal zone |
| cer1.S | cer, cer-1, Cerberus, dand4, LOC108713989, tCerberus, xcer, xcer-1, xcer1 | X. laevis | Throughout NF stage 11 | dorsal marginal zone |
| otx2.S | otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 | X. laevis | Throughout NF stage 20 | pre-chordal neural plate |
| gdf10.L | bmp-3b, bmp3b, gdf-10 | X. laevis | Throughout NF stage 20 | neural plate border |
| hoxc9-like.L | X. laevis | Throughout NF stage 28 | spinal cord posterior | |
| cdx2.L | cad2, cdx-2, cdx3-like, xcad2, Xcaudal2 | X. laevis | Throughout NF stage 28 | tail bud post-anal gut |
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