Fig. 5. Construction and effectiveness of a hormoneinducible XTbx5 protein. (A) Schematic diagram of wildtype and dominant negative XTbx5 constructs. The T domain is in red, the engrailed repressor domain is depicted in black and the GR domain is in blue. (B) Wild-type stage 35 embryo with dexamethasone added at the 4 cell stage. (C) Stage 35 embryo injected with 1 ng of XTbx5-EnR mRNA into the dorsal marginal zone at the 4-cell stage. (D) Stage 35 embryo injected with 1 ng of XTbx5-EnR-GR mRNA and no dexamethasone added. (E) Stage 35 embryo injected with 1 ng of XTbx5-EnR-GR mRNA and dexamethasone added immediately after injections. (F) Stage 35 embryo injected with 1 ng of XTbx5-EnR-GR mRNA and dexamethasone added at stage 14/15. In B-F, the inset in the lower left corner shows a single example of a stage 17 embryo.
Image published in: Horb ME and Thomsen GH (1999)
Copyright © 1999. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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