Fig. 6. Embryonic phenotypes generated by a dominant negative XTbx5 protein. (A) Wild-type stage 45 embryo. Arrow points to the heart. Notice how the heart extends to the edge of the pericardial cavity and is near the ventral surface of the gut. (B-F) Typical phenotypes caused by XTbx5-EnR-GR. In each case, embryos were injected dorsally at the 4-cell stage with 1 ng of mRNA and treated with dexamethasone at stage 14/15. (B,C) Examples of the reduced heart phenotype. Arrow points to the small heart present in this class of embryo. (D-F) Examples of the beating nub or heartless phenotype. This phenotype is typified by the absence of a morphologically recognized heart. Sometimes, some small amount of beating tissue is present in the dorsal portion of the pericardial cavity. In B-F, notice the bloated pericardial cavity and improper gut formation in the injected embryos. In addition, the eyes are smaller in size. Arrowhead in B and E points to regions where blood cells have pooled.
Image published in: Horb ME and Thomsen GH (1999)
Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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