Fig. 8. Additive effects of Xvent genes in gainand loss-of-function. (A) Xvent genes ventralize mesoderm in an additive fashion. Embryos were either non-injected (Co) or microinjected radially at the 4- to 8-cell stage with Xvent-1 (range 0.125-0.25 ng/blastomere) or Xvent-2 mRNA (range 0.37-075 ng/blastomere), or injected with a mixture (same range of both). The average dorsoanterior index (Kao and Elinson, 1988) of embryos was 4.8 (uninjected control, n=22), 4.4 (Xvent-1, n=34), 3.0 (Xvent- 2, n=47) and 2.6 (Xvent-1 + Xvent-2, n=33). (B) Xvent genes function in an additive fashion in downregulation of dorsal and upregulation of ventral marker genes in DMZ. Embryos were either non-injected (Co), or microinjected radially at the 4- and 8-cell stage with the doses indicated in ng/blastomere with Xvent-1 or Xvent-2 mRNAs, or injected with a mixture. Control (Co) VMZ and DMZs were explanted at early gastrula stage and fixed immediately for total RNA preparation. Expression of marker genes indicated was assayed by RT-PCR. (C) VPXvent-1/2 mRNA injections induce dorsal marker genes additively. Embryos were either non-injected (Co), or microinjected radially at the 4- to 8-cell stage with the doses indicated in ng/blastomere with VPXvent-1 or VPXvent-2 mRNAs, or injected with a mixture. Control (Co) DMZ and VMZs were explanted at early gastrula stage and fixed immediately for total RNA preparation. Expression of marker genes indicated was assayed by RT-PCR. Histone H4 was used for normalisation.
Image published in: Onichtchouk D et al. (1998)
Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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