Fig. 5. Brat induces mesodermal tissues. (A) Control-injected animal caps form solid balls of atypical epidermis. (B) At low doses, 50 pg, Brat-injected animal caps form vesicles, characteristic of ventral mesoderm. Caps in (A) and (B) were scored at stage 28. (C-F) Whole-mount staining for muscle with the 12/101 antibody. (C) Sibling stage 24 whole embryo. Muscle tissue is stained in the segmented somites. (D) CS2-injected animal caps, 250 pg mRNA. No muscle tissue formed, n=11. (E) Brat-injected animal caps, 500 pg mRNA. Muscle formed in 33% of the injected caps, n=27. (F) BVg1-injected animal caps, 5 pg mRNA. Muscle staining is seen in all caps, n=22.
Image published in: Horb ME and Thomsen GH (1997)
Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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