Fig. 3. Expression pattern of X-Delta-2 transcripts in Xenopus embryos. In this and other figures, embryos and sections are oriented with anterior on the left. (A-C) Embryos stained in whole mount for the expression of X-Delta-2 RNA at neural plate stage (A; dorsal view, stage 14), neurulae stage (B; dorsal view, stage 18), and tailbud stage (C; side view, stage 28). Note that X-Delta-2 is expressed within the paraxial mesoderm within stripes of cells that are located progressively more posterior as development progresses (arrows). Note also that X-Delta-2 expression occurs in a large domain in the tailbud. (D) Longitudinal section through neurulae embryos (stage 18), showing just one side of the paraxial mesoderm. The three somites that have formed (labeled a, b and c; demarcated by arrowheads) do not express X-Delta-2 at detectable levels. Within the presomitic mesoderm, however, X-Delta-2 RNA is expressed in stripes of cells, where the spacing between each strip (denoted by arrows) corresponds to about ten cells, and thus the width of a prospective somite, or somitomere (labeled 1-4; Jacobson and Meier, 1986). Note that X-Delta-2 expression in the youngest somitomeres (labeled 3 and 4), is broad but then undergoes a refinement, localizing to the anterior edge of the first somitomere (labeled 1). (E,F) Dorsal view of myogenesis in the paraxial mesoderm of neural tube stage embryos (stage 18) as revealed by the expression of cardiac actin (E) and of MyoD (F). (G) Expression of X-Notch-1 RNA in the presomitic mesoderm (double arrow) in a cleared, early tadpole embryo (stage 24), as shown by whole-mount in situ hybridization. (H) Dorsal view of an early neural plate stage embryo (stage 12.5), double-labeled for the expression of cardiac actin RNA (dark blue) and for X-Delta-2 RNA (light blue). Note that the X-Delta-2 staining (arrow) extends outside the myogenic domain marked by cardiac actin staining.
Image published in: Jen WC et al. (1997)
Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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