FIG. 4. Mammalian LEF-1 can bind to the sequence from -125 to -63. Embryos were injected with 1 ng LEF-1 mRNA, 1 ng LEF- 1DN mRNA, or 1 ng Xwnt mRNA at the single-cell stage. They were harvested at gastrulation and extracts were incubated with a radiolabeled copy of the sequences between -125 and -63 of the Xnr3 promoter. A novel shifted complex is detected in extracts from embryos injected with LEF-1 mRNA (lane 2). A complex of greater mobility is detected in extracts from embryos injected with LEF-1D56 mRNA (lane 3), indicating that mammalian protein can bind these sequences directly.
Image published in: McKendry R et al. (1997)
Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V.
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