Fig. 6. 3,5,3 -triiodothyronine (T3) upregulates Xenopus basic transcription element binding protein (xBTEB) mRNA in the brain (diencephalon), intestine and tail of premetamorphic Xenopus laevis tadpoles. Stage 52 X. laevis tadpoles were treated with either 50 nM (for brain and intestine blots) or 200 nM T3 (for tail blot) in the aquarium water and tissues were collected at various times thereafter and analyzed by northern blot. Representative northern blots of xBTEB mRNA expression in the (a) diencephalon, (b) intestine and (c) tail, respectively. Graphs show the densitometric quantitation of northern blot data (n = 3 per tissue). Bars in the graphs represent the mean mRNA level, expressed as a percentage of maximal expression (n = 3 blots per tissue). (d) xBTEB gene expression during metamorphosis depends on thyroid hormone. Northern blot of xBTEB mRNA expression in the diencephalon of prometamorphic (Nieuwkoop and Faber stage 58) X. laevis tadpoles treated with the goitrogen methimazole (1 mM) for 7 days.
Image published in: Hoopfer ED et al. (2002)
Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons.
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