Fig. 6. CASZ1 and CHD5 are required for myocardial tissue adhesion prior to cardiac looping and chamber formation. (A-X) Control, CHD5-depleted and CASZ1-depleted CA-GFP embryos were examined for expression of the tight junction markers ZO-1 (A-L) and claudin-5 (M-X). Expression of both ZO-1 and claudin-5 is reduced and appears diffuse and unorganized in transverse sections of CHD5- and CASZ1-depleted hearts (stage 33). Phenotypes represented across multiple sampled embryos (n≥4). White boxes in A,B,E,F,I,J,M,N,Q,R,U,V correspond to magnified images in C,D,G,H,K,L,O,P,S,T,W,X, respectively. Merge represents ZO-1 or claudin-5 (red), DAPI (blue) and CA-GFP (green). Scale bars: 100 µm in A,B,E,F,I,J,M,N,Q,R,U,V; 20 µm in C,D,G,H,K,L,O,P,S,T,W,X.
Image published in: Sojka S et al. (2014)
Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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