Figure 2. MPC088 agonist activity in oocytes expressing α1β2γ2 GABAARsa: Representative responses to MPC088 obtained in a single experiment. b: Concentration-response functions (mean ± SD) for trans- and cis-dominant MPC088 (n = 6) and propofol (n = 6). In each of the MPC088 experiments, the cis-dominant form was generated by UV light during static bathing. Amplitudes normalized to the 100 μM GABA response. Inset: Sensitivity (mean ± SD) of the MPC088 (15 μM) response to 100 μM PTX (n = 4), 100 μM bicuculline (n = 4) and 30 μM gabazine (n = 4). c: Photo-regulation of the response to 15 μM MPC088. d: Normalized current amplitudes (mean ± SD) determined in the experiment of c and in 3 others of similar design. Amplitudes in each experiment normalized to that elicited by 3 μM GABA. Numbers beneath the waveform in c denote amplitude determination conditions for the experiment shown in c. Repeated-measures ANOVA conducted on the aggregate data yielded F(6,18) = 68.988, p < 0.001. Post-hoc paired-sample t-tests corrected for multiple comparisons indicated significant differences produced by the initial UV, the initial High-Vis, the second UV, and the second High-Vis illuminations (p ≤ 0.010 for amplitude group 2 vs. group 1, for 4 vs. 3, for 5 vs. 4, and for 6 vs. 5, respectively); amplitude groups 7 and 1 did not differ significantly (p = 0.96). e: Representative waveform obtained with repeated presentation of UV light during continuous High-Vis illumination (static bathing in MPC088-supplemented Ringer).
Image published in: Yue L et al. (2012)
Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder.
Permanent Image Page
Printer Friendly View
XB-IMG-137007