Larger Image FIG. 7. Localization of XLPOU 1 by whole mount in situ hybridization. Whole, fixed embryos were hybridized to either sense or antisense digoxigenin-labeled riboprobes of XLPOU 1 OP at stage 15 (control is below). The dark blue reaction product representing hybridization can easily be distinguished from the dark brown pigment found in wild-type embryos. Embryos hybridized to the sense probe (controls) were lightly stained in comparison to embryos hybridized to the antisense probe. The anterior (A) and posterior (P) ends of the embryo, neural plate (np), and neural folds (nf) are indicated. Image published in: Agarwal VR and Sato SM (1991) Copyright © 1991. Image reproduced with permission of the Publisher, Elsevier B. V.
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