Fig. 9. Intracellular calcium increase is required for neural induction mediated by attenuation of BMP signals. Expression of the pan-neural marker NCAM in the animal caps was measured by RT-PCR. Stage 8-9 animal caps treated with noggin (A) or dissociated caps (B) differentiated into neural cells expressing NCAM, whereas R(+)BayK pretreated animal caps before noggin induction (R(+)BayK/noggin) and BAPTA-AM loaded caps before dissociation (Dissociated+BAPTA) show a dramatic reduction in NCAM expression. Intact cap, animal caps not treated with noggin or nor dissociated; sibling control embryos served as positive control (Embryo) and PCR on the same RNA without reverse transcription was done to check the absence of genomic DNA (-RT). ODC is a control with ornithine decarboxylase.
Image published in: Leclerc C et al. (2000)
Copyright © 2000. Image reproduced with permission of the Publisher, The Company of Biologists Ltd.
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