Figure S8. Knockdown of PN1 promotes mesoderm and suppresses ectoderm formation in an HtrA1- and FGF-dependent manner Embryos were separately injected with MOs and mRNAs into the animal pole at the 4-cell stage. Hemisectioned early gastrula embryos are shown after whole-mount in situ hybridization for Xbra (A-F) and Foxi1e (A’-F’). (A-B’) PN1-MO, but not control-MO, induces ectopic Xbra and reduction of Foxi1e expression in deep cells of the animal hemisphere (brackets in insets depict animal cap). (C-D’) Injections of 200 pg HtrA1 and 2 pg Fgf4 mRNA induce ectopic Xbra at the expense of Foxi1e expression in the inner layer of the animal cap (insets). (E-F’) Injections of HtrA1-MO and 80 pg XFD mRNA restore normal expression of Xbra and Foxi1e in PN1-morphant embryos. Total injected MO amount was 60 ng (+20 ng HtrA1-MO). The frequency of the indicated phenotypes was A, 136/144; A’, 50/59; B, 148/177; B’, 50/69; C, 21/21; C’, 47/57; D, 79/79; D’, 93/97; E, 66/90; E’, 52/56; F, 72/83; F’, 45/51.
Image published in: Acosta H et al. (2015)
Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| tbxt.S | bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury | X. laevis | Throughout NF stage 10.5 | mesoderm involuted dorsal mesoderm involuted ventral mesoderm involuting marginal zone |
| foxi1.L | ectodermally-expressed mesendoderm antagonist, ema, FoxI1e, foxi1e, foxi3, Xema, xfoxi1 | X. laevis | Throughout NF stage 11 | sensorial layer of neurectoderm |
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