Figure S3. (A) Schematic of fezf2 MO design and knockdown efficiency. The MO targets the exon3-intron3 junction (above) and semi-quantitative PCR analysis shows mature fezf2 mRNA levels in stage 24 embryos (below), injected with control MO (1) or fezf2 MO (2). Black arrow pair indicates the position of validation primers for RTPCR analysis, the forward primer overlaps the exon3-exon4 junction. (B) qPCR results, n=3 replicates. In both cases, the ribosomal protein L8 (rpl8) gene was used as an internal control.
Image published in: Zhang S et al. (2014)
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