Fig. 2. Wdpcp knockdown caused severe membrane blebbing exclusively at the basolateral membrane. (A) The apical plasma membranes in control Xenopus epidermal epithelium, showing tight cell–cell contacts. (a0) The basolateral membranes of cells shown in panel (A) are also remain stably connected (B). The apical membrane of Wdpcp morphant tissue was indistinguishable from the control apical membrane. (b0) Wdpcp morphants displayed severe blebbing of the basolateral membranes. (C) X–Z projection of control epithelium. Green arrowheads indicate apical junctions. Blue arrowheads indicate basolateral membrane. (D) X–Z projection of Wdpcp morphant epithelium. Blue arrowheads indicate blebbing basolateral membrane while the apical junction is quite stable (green arrowheads). The scale bars are 10 lm. (c0 and d0) The drawing indicating the optical sectioning points for confocal imaging of (A and B) (Green lines) or (a0 and b0) (Blue lines).
Image published in: Park TJ et al. (2015)
Copyright © 2015. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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