Figure S7. Vgll3 is required for the formation of neural crest derivatives. Embryos were injected with 40 ng of v3MO (or cMO) or 1 ng of vgll3 mRNA (or gfp mRNA) and fixed at stage 47 before alcian blue staining (A-I). (A, B, C, D) The formation of cranial cartilage is impaired when vgll3 is knocked-down when compared to control MO injected embryo [A compared to D (embryos injected with cMO show no alteration)]. This is more obvious on dissected embryos that revealed on the injected side a high rate of cranial cartilage hypoplasia including severe loss of Meckel’s (MC), ceratohyal (CH) and branchial cartilages (Br) (100%, n=15, Fig. 6B,C). (E, F) Transversal sections of paraffin embedded tissues showed that the ceratohyal cartilage on the injected side was shorter than on the uninjected control side (Fig. 6E) and displayed numerous smaller chondrocytes (Fig. 6F, enlarged view of E indicated in the square line. Scale bars represent 500 μm (E) and 130 μm (F). (G, H, I, J) Embryos injected with vgll3 mRNA showed an impaired cartilage development with the ceratohyal cartilage being severely disorganized (100%, n=15, Fig. 6G, H). In control experiments, embryos injected with gfp mRNA showed no change in cartilage head morphology (Fig. 6I, J). ey, eye. Ventral views with anterior to the top (A, D, G, I) and dorsal views (J). (*) injected side. Midline embryo is indicated by a dotted line.
Image published in: Simon E et al. (2017)
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