Figure S3. Related to Fig. 2; Expression of gastrulation genes in rapgef5 morphants. (A) At stage 10, expression of numerous gastrulation markers (gsc, chordin, fgf8, otx2, vent, t, not and noggin) are less affected by depletion of Rapgef5 compared to foxj1 or xnr3 (See Fig. 2A). Vegetal views with dorsal to the top. (B) Injection of a control MO or a control CRISPR (targeting tyrosinase) does not affect foxj1 expression (C) Co-injection of human RAPGEF5 mRNA can rescue the loss of foxj1 expression in Rapgef5 morphants, demonstrating the specificity of the MO. Triple asterisks indicate P<0.005.
Image published in: Griffin JN et al. (2018)
Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| foxj1.L | foxj1-a, foxj1-b, hfh-4, hfh4, xfoxj1 | X. laevis | Throughout NF stage 10 | dorsal marginal zone |
| gsc.L | goosecoid, gsc-a, gsc-b, Xgsc | X. laevis | Throughout NF stage 10 | dorsal marginal zone |
| chrd.L | chd, chordin, chrd.1, LOC108716939, X-chordin | X. laevis | Throughout NF stage 10 | dorsal marginal zone |
| fgf8.L | AIGF, FGF-8, fgf-8b, fgf8a, fgf8b, MGC79739, Xfgf-8, Xfgf8 | X. laevis | Throughout NF stage 10 | marginal zone |
| otx2.L | otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 | X. laevis | Throughout NF stage 10 | dorsal marginal zone |
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