Figure S2: Conservation of the C-terminal Tail of Pkd1. (A) ClustalW alignment of the intracellular domain of Polycystin-1 protein from human (GenBank Accession #NP_000287.3) and Xenopus tropicalis (GenBank Accession #XP_002932517.1). Four previously described domains were analyzed. The G-protein binding signature (GBD) is based on the analysis by Parnell et al. (1998). It is indicated in yellow and the domain-identifying amino acids (BB….BBxxB) are bolded. This sequence overlaps with the Nuclear Localization Sequence (NLS) described in Chauvet et al. (2004) and is indicated by a black line above the human sequence. The PEST sequences were identified using epestfind. The optimal and predicted domains are indicated in green and turquoise, respectively; the key amino acids identified in Low et al. (2006) are indicated in bold. The coiled-coil domain were identified using Paircoil2 and are labeled in red. (B,C) Immunofluorescence analysis of HEK293T cells transiently transfected with Pkd1-CTT-Myc (B) or Pkd1-CTTΔGBD-Myc (C). Nuclei were counterstained with DAPI (blue). Insets show close-up of individual cells.
Image published in: Zhang B et al. (2018)
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