Fig. 6: Depletion of Nosip leads to reduced marker gene expression during eye development. A WMISH at stage 13 shows reduced expression of eye specific marker genes such as rax and pax6 on the Nosip MO injected side (black arrowheads). In contrast, the pan-neural marker gene sox3 is unaffected. Un-injected and Control MO injected sides showed normal marker gene expression. Anterior views are shown. B Statistical evaluation of data in A. C At stage 23, depletion of Nosip reduces the expression of the eye-specific marker genes rax, pax6 and otx2 (black arrowheads). Un-injected and Control MO injected sides showed normal marker gene expression. Anterior views are shown. D Quantification of the data in C. Abbreviations: N, number of evaluated embryos in total; n, number of independent experiments, n.s., not significant. Error bars visualize standard error of the means (s. e. m.); * p≤0.05. Pvalues are calculated by a nonparametric, one-tailed Mann-Whitney rank sum test.
Image published in: Flach H et al. (2018)
Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| rax.L | rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A | X. laevis | Throughout NF stage 13 | optic field |
| pax6.L | an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 | X. laevis | Throughout NF stage 13 | optic field neural plate |
| sox3.L | Sry, Xlsox3, xSox3, Xtsox3 | X. laevis | Throughout NF stage 13 | optic field neural plate |
| rax.L | rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A | X. laevis | Throughout NF stage 23 | optic vesicle |
| pax6.L | an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 | X. laevis | Throughout NF stage 23 | optic vesicle neural tube |
| otx2.L | otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 | X. laevis | Throughout NF stage 23 | optic vesicle anterior neural tube |
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