Fig. 1. Knockdown of ADAM13 or ADAM19 inhibits Wnt signaling and NC specification but not NPB formation. (A) cDNA sequences of adam12 and adam19 showing the translation start sites (red) and targets of MOs (colored boxes). (B) Knockdown (KD) of ADAM13 or ADAM19 inhibits head cartilage formation. Transgenic X. tropicalis snail2- eGFP embryos were injected in one anterodorsal (D1) blastomere at the eight-cell stage with the indicated MO (1.5 ng each) cultured to stage ∼46 and imaged for eGFP expression. One representative embryo of each injected group (ventral view) is shown in the upper panels and the results of five independent experiments are shown in the lower panel. (C,D) Effects of ADAM13 or ADAM19 KD on NC and NPB marker expression. Wild-type embryos were injected in one blastomere at the two-cell stage with the indicated MO (6 ng each) and cultured to stage ∼12.5. Embryos were processed for in situ hybridization for the indicated genes. (E) KD of ADAM13 or ADAM19 inhibits endogenous Wnt signaling. Transgenic Wnt reporter embryos were injected as in C,D, cultured to stage ∼12.5 and imaged for eGFP expression. White arrowheads indicate the Wnt signal at the NPB. Embryos in C-E are shown in dorsal view, with anterior at the top. Red asterisks indicate the injected side. CT, control MO; n, number of embryos scored; N, number of independent experiments performed; ***P<0.001; NS, not significant.
Image published in: Li J et al. (2018)
Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| snai2 | slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 | X. tropicalis | Throughout NF stage 12.5 | neural plate border |
| sox9 | cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 | X. tropicalis | Throughout NF stage 12.5 | neural plate border |
| foxd3 | fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 | X. tropicalis | Throughout NF stage 12.5 | neural plate border |
| pax3 | cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 | X. tropicalis | Throughout NF stage 12.5 | neural plate border |
| zic1 | odd-paired-like, opl, Zic-r1, Zic-related-1 | X. tropicalis | Throughout NF stage 12.5 | neural plate border |
| msx1 | hox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1 | X. tropicalis | Throughout NF stage 12.5 | neural plate border |
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