Supplemental Figure 2: Characterization of Dsp expression. A) RT-PCR of Dsp at embryonic stages as well positive controls in the adult skin and heart. RT+ = reverse transcriptase was added, RT- = reverse transcriptase was not added. Primer sequences available upon request. B-E''') Immunofluorescence of Dsp (green) in the epidermis from various embryonic stages. Dapi (blue) and beta-catenin (pink) labeling were used as a counter stain. F-F''') A 3D rendering of images as shown in E-E''' were created and then rotated 90 degrees. G-G''') st. 30 where only the inner epidermal layer of cells are shown. All scale bars = 40µm
Image published in: Bharathan NK and Dickinson AJG (2019)
Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| dsp.L | X. laevis | Sometime during NF stage 10 to NF stage 11 | ectoderm | |
| dsp.L | X. laevis | Sometime during NF stage 19 to NF stage 20 | epidermis | |
| dsp.L | X. laevis | Sometime during NF stage 24 to NF stage 25 | epidermis | |
| dsp.L | X. laevis | Throughout NF stage 32 | epidermis outer layer | |
| ctnnb1.L | B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin | X. laevis | Sometime during NF stage 10 to NF stage 11 | ectoderm |
| ctnnb1.L | B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin | X. laevis | Sometime during NF stage 19 to NF stage 20 | epidermis |
| ctnnb1.L | B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin | X. laevis | Sometime during NF stage 24 to NF stage 25 | epidermis |
| ctnnb1.L | B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin | X. laevis | Throughout NF stage 32 | epidermis outer layer epidermis inner layer |
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