Larger Image FIG. 4. Milestones of early cardiac development in Xenopus (continued). Embryos were double labeled with anti-cardiac troponin T (CT3) (red) and anti-fibrillin (JB3) (green) and optically sectioned. For each stage, the set of optical sections (35 to 53 sections 5 to 7 um thick) was digitally compiled in increments of 6 to 9 sections. Resulting ventral views of optical thick sections are presented. By stage 35 (A and B) the myocardial wall is closed and the heart tube has become twisted in a S shape; the chambers are now distinct. Stage 41 (C and D) The myocardium thickens and develops trabeculae; the atrium is posterior to the ventricle. At stage 44 (E and F) the single atrium begins to be partitioned by the forming septum and is pushed slightly anterior to the ventricle. Scale bar, 100um. Image published in: Kolker SJ et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V.
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