Figure 6. Expression of RA-modifying genes during hindlimb regeneration. Dark blue stain indicates expression, white arrowheads mark the level of amputation; tissue to the right of this is regrowth. A–C:Cyp26b is expressed strongly in mesenchymal blastema cells, but is absent from the AEC. D–F:Crabp2 is also strongly upregulated in the blastema although it appears absent from the distal tip (white arrow in E). D′–F′: Expression of Crabp2 in non-regenerating, transgenic N1 tadpole hindlimbs to show residual expression. G–I:Raldh2 is not expressed in regenerating tissue but note that it is expressed in the anterior stump tissue (marked with a white asterisk) providing a potential source of RA to the blastema. Limbs are viewed from the dorsal side and oriented with proximal to the left and posterior uppermost, the tadpole's head is to the left. Scale bar in I = 0.5 mm (applies to all panels).
Image published in: McEwan J et al. (2011)
Copyright © 2011. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| cyp26b1.L | X. laevis | Sometime during NF stage 52 to NF stage 56 | distal mesenchyme regenerating tissue blastema wound epidermis | |
| crabp2.L | xCRABP | X. laevis | Sometime during NF stage 52 to NF stage 56 | distal mesenchyme blastema mesenchyme regenerating tissue anterior posterior |
| aldh1a2.L | raldh-2, raldh2, XRaldh2 | X. laevis | Sometime during NF stage 52 to NF stage 56 | blastema mesenchyme anterior wound epidermis |
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