Fig. 5. Depletion of chordin and tsg reduces dorsal tissues and results in a cooperative expansion of ventral tissues. Embryos were injected at the two-cell stage with tsg MO (B, F, J, N, R, V, Z, DD), chordin MO (C, G, K, O, S, W, AA, EE), or both MOs (D, H, L, P, T, X, BB, FF) and compared to uninjected sibling control embryos (A, E, I, M, Q, U, Y, CC). Embryos were assayed for expression of the ventral markers BAMBI (E H) and sizzled (I L) and the somite marker myoD (M P). Embryos were also assayed for expression of several regional specific markers of the neural plate: eomesodermin (Q), otx2 (U), en-2 (YB) and krox-20 (CC FF). All embryos are between stages 22 25. Embryos stained for expression of myoD (M P) and krox -20 (CC FF) are viewed dorsally with anterior to the left, all others are viewed laterally with anterior to the left.
Image published in: Wills A et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| bambi | MGC89501 | X. tropicalis | Sometime during NF stage 22 to NF stage 25 | anterior ventral blood island |
| szl | frzb3, secreted frizzled, sizzled, xszl | X. tropicalis | Sometime during NF stage 22 to NF stage 25 | ventral blood island |
| myod1 | mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD | X. tropicalis | Sometime during NF stage 25 to NF stage 26 | paraxial mesoderm presomitic mesoderm |
| eomes | eomesodermin, tbr2, xeomes | X. tropicalis | Sometime during NF stage 25 to NF stage 26 | forebrain telencephalon dorsal |
| otx2 | otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 | X. tropicalis | Sometime during NF stage 25 to NF stage 26 | forebrain olfactory placode optic vesicle midbrain neuroectoderm |
| en2 | En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 | X. tropicalis | Sometime during NF stage 25 to NF stage 26 | brain midbrain midbrain-hindbrain boundary |
| egr2 | EGR-2, Krox-20, krox20, XKr20, XKrox-20 | X. tropicalis | Sometime during NF stage 25 to NF stage 26 | hindbrain rhombomere R3 rhombomere R5 anterior branchial crest |
Image source: Published
Permanent Image Page
Printer Friendly View
XB-IMG-74200