Fig. 3. Localisation of maternal Apod mRNA throughout oogenesis and early cleavage stages in Xenopus. Apod mRNA was visualised by in situ hybridisation to previously sectioned material using DIG-labelled antisense riboprobes (A-D) or sense probes as negative controls (E). Cells are orientated such that the animal pole is uppermost. (A) Stage 3 oocyte showing presence of Apod mRNA in both the vegetal cortex and yolk mass. (B) Stage 4 oocyte. (C) Mature stage 6 oocyte showing the particulate localisation of Apod mRNA in the vegetal yolk mass. The mRNA is now localised in a more subequatorial position. (D) Egg showing more diffuse localisation of Apod mRNA, still distributed in the vegetal yolk mass. (E) Egg hybridised with a sense probe showing very faint non-specific staining throughout the animal hemisphere only. A and B are shown at a greater magnification than C-E for convenient comparison. (gv, germinal vesicle)
Image published in: Stennard F et al. (1996)
Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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