Fig. 4. Expression analysis of pronephric markers. (A–H′) Whole mount in situ hybridization analysis of uninjected control and xBic-C-MO1 + 2-injected embryos at stage 38. (A, A′) Nephrin; (B, B′) xSGLT-1K; (C, C′) NKCC2; (D, D′) xNBC1; (E, E′) CAII; (F, F′) ClC-K; (G, G′) NCC; (H, H′) ROMK. (I) Schematic diagram of the pronephric marker genes used. Arrows point to the late distal pronephric tubule, arrowheads indicate the pronephric duct.
Image published in: Tran U et al. (2007)
Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| nphs1.S | nephrin | X. laevis | Throughout NF stage 37 and 38 | glomus |
| slc5a1.2.S | D22S675, sglt-1, sglt-1k, sglt1 | X. laevis | Throughout NF stage 37 and 38 | pronephric kidney early proximal tubule late proximal tubule |
| slc12a1.L | Na-K-2Cl cotransporter, NKCC-2, NKCC2, XNKCC2 | X. laevis | Throughout NF stage 37 and 38 | pronephric kidney pronephric tubule early distal tubule |
| slc4a4.L | hhNMC, hnbc1, nbc1, nbc2, nbce1, pNBC, slc4a5, XNBC1 | X. laevis | Throughout NF stage 37 and 38 | pronephric kidney pronephric tubule early proximal tubule late distal tubule |
| ca2.L | CA-2, caII, car2, carbonic anhydrase II, carbonic anhydrase type 2 | X. laevis | Throughout NF stage 37 and 38 | pronephric kidney pronephric tubule early proximal tubule late distal tubule ciliated epidermal cell |
| clcnkb.L | ClC-k, clc-kb, clckb, clcnka, clk-k2, xClC-k | X. laevis | Throughout NF stage 37 and 38 | pronephric kidney pronephric duct pronephric tubule early distal tubule late distal tubule |
| slc12a3.L | ncc, ncct, tsc | X. laevis | Throughout NF stage 37 and 38 | pronephric duct late distal tubule |
| kcnj1.L | kir1.1, MGC68935, ROMK, romk1 | X. laevis | Throughout NF stage 37 and 38 | pronephric kidney pronephric duct pronephric tubule early distal tubule late distal tubule |
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