Larger Image Figure 1. ATP4a Is Symmetrically Expressed and Required Post-MBT for LR Development(A�E) Whole-mount in situ hybridization of staged embryos at the (A) two-cell, (B) four-cell, and (C) 32-cell stages. Note that ATP4a mRNA was enriched in the animal cytoplasm, as shown in the bisected four-cell embryo in (B), viewed from the inner surface in (B′) and (B�), but symmetrically expressed with respect to the LR axis. (D) Zygotic ATP4a expression in the stomach of a stage (st.) 45 tadpole; see plane of section in (D′) indicated by dashed line in (D). Arrowhead indicates gastric epithelium in blow-up. (E) Four-cell embryo stained with sense control RNA.(F and G) (F) Pitx2c expression and (G) organ situs in wild-type embryos at tadpole stages (26�32) and in specimens injected or treated as specified. The image in (D′) was reconstructed from several images taken from the same histological section. ∗∗Highly significant. ∗∗∗Very highly significant. ns, not significant; n, number of embryos analyzed; wt, wild-type.See also Figure S1. Image published in: Walentek P et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue atp4a.L alpha H-K, gastric H(+)-K(+)-ATPase alpha-subunit, LOC108696872, MGC69393 X. laevis Sometime during NF stage 2 (2-cell) to NF stage 6 (32-cell) animal cap animal hemisphere animal pole atp4a.L alpha H-K, gastric H(+)-K(+)-ATPase alpha-subunit, LOC108696872, MGC69393 X. laevis Throughout NF stage 45 stomach alimentary system gut epithelium Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + atp4a MO + NF32 (in situ hybridization) fig.1.f Expression Phenotype decreased amount pitx2.S expression in lateral plate mesoderm Xla Wt + atp4a MO + NF32 (in situ hybridization) fig.1.f Expression Phenotype decreased amount pitx2.S expression in lateral plate mesoderm Xla Wt + SCH28080 + NF32 (in situ hybridization) fig.1.f Expression Phenotype decreased amount pitx2.S expression in lateral plate mesoderm Xla Wt + atp4a MO + NF45 (morphology) fig.1.g Anatomical Phenotype abnormally localised gall bladder abnormally localised outflow tract absent gall bladder absent outflow tract Xla Wt + SCH28080 + NF45 (morphology) fig.1.g Anatomical Phenotype abnormally localised gall bladder absent gall bladder Xla Wt + SCH28080 + NF45 (morphology) fig.1.g Anatomical Phenotype abnormally localised gall bladder abnormally localised outflow tract absent gall bladder absent outflow tract Permanent Image Page Printer Friendly View XB-IMG-82870