Figure 2. Establishment of the HSC Program and Arterial Specification of the DA Is Abolished in Tel1-Depleted Embryos (A) In situ hybridization analysis showing that expression of hematopoietic genes in the ventral wall of the DA (arrows) is absent in Tel1 morphants. In contrast, expression in embryonic erythrocytes (Runx1, Scl, and Lmo2, arrowheads) and myeloid cells (SpiB and Gfi1, arrowheads) is still observed. (B) Expression analysis showing that the DA (arrows) of Tel1 morphants does not express the arterial affiliated genes, Notch4, Dll4, and EphrinB2, nor the Notch target, Gata2. (C) Endothelial expression of Erg1 reveals the presence of DA precursors (arrows) coalescing in the midline of Tel1 morphants. All embryos were hybridized as whole mounts and are shown in lateral view, with anterior to the left and dorsal to the top. Stages of development are as indicated. The numbers of embryos represented by each panel, out of the number analyzed, are indicated in the top right corner. See also Figure S1 and Table S1.
Image published in: Ciau-Uitz A et al. (2010)
Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| runx1.L | aml, aml-1, aml1, aml1 oncogene, aml1-evi-1, amlcr1, cbfa2, evi-1, LOC108709238, pebp2ab, Runx-1, Xaml, Xaml1 | X. laevis | Sometime during NF stage 41 to NF stage 43 | dorsal aorta ventral |
| tal1.L | bHLHa17, stem cell leukaemia, tal-1, tcl5, xscl | X. laevis | Sometime during NF stage 41 to NF stage 43 | dorsal aorta ventral blood island ventral blood hematopoietic stem cell |
| lmo2.L | LMO-2, xlmo-2, xlmo2 | X. laevis | Sometime during NF stage 41 to NF stage 43 | dorsal aorta ventral |
| runx1.L | aml, aml-1, aml1, aml1 oncogene, aml1-evi-1, amlcr1, cbfa2, evi-1, LOC108709238, pebp2ab, Runx-1, Xaml, Xaml1 | X. laevis | Sometime during NF stage 41 to NF stage 43 | blood |
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