Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
EMBO Rep September 6, 2021; 22 (9): e50932.
Show Gene links Show Anatomy links

Foxm1 regulates neural progenitor fate during spinal cord regeneration.

Pelzer D , Phipps LS , Thuret R , Gallardo-Dodd CJ , Baker SM , Dorey K .

Xenopus tadpoles have the ability to regenerate their tails upon amputation. Although some of the molecular and cellular mechanisms that globally regulate tail regeneration have been characterised, tissue-specific response to injury remains poorly understood. Using a combination of bulk and single-cell RNA sequencing on isolated spinal cords before and after amputation, we identify a number of genes specifically expressed in the spinal cord during regeneration. We show that Foxm1, a transcription factor known to promote proliferation, is essential for spinal cord regeneration. Surprisingly, Foxm1 does not control the cell cycle length of neural progenitors but regulates their fate after division. In foxm1-/- tadpoles, we observe a reduction in the number of neurons in the regenerating spinal cord, suggesting that neuronal differentiation is necessary for the regenerative process. Altogether, our data uncover a spinal cord-specific response to injury and reveal a new role for neuronal differentiation during regeneration.

PubMed ID: 34427977
PMC ID: PMC8419688
Article link: EMBO Rep
Grant support: [+]

Species referenced: Xenopus tropicalis
Genes referenced: aurka aurkb ccna2 ccnb1 ccnb2 ccnb3 ccnd1 ccne1 cdc20 cdc25a cdc25b cdc25c cdk1 cdkn1b cenpi cenpn cfd ep400 erbb2 foxm1 hdac1 hgf lep mmp2 myc myt1 neurod1 pcna rabl6 shh snai1 snai2 snap25 sox2 sox3 tbx2 tgfb1 tubb2b twist2 xbp1
GO keywords: cell cycle [+]
Antibodies: BrdU Ab9 Casp3 Ab1 H3f3a Ab9 Pcna Ab5
Morpholinos: foxm1 MO2 foxm1 MO3
gRNAs referenced: foxm1 gRNA1

Article Images: [+] show captions
References [+] :
Arai, Neural stem and progenitor cells shorten S-phase on commitment to neuron production. 2021, Pubmed