Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Endocrinol
2017 Jun 15;448:87-97. doi: 10.1016/j.mce.2017.03.028.
Show Gene links
Show Anatomy links
Paxillin and embryonic PolyAdenylation Binding Protein (ePABP) engage to regulate androgen-dependent Xenopus laevis oocyte maturation - A model of kinase-dependent regulation of protein expression.
???displayArticle.abstract???
Steroid-triggered Xenopus laevis oocyte maturation is an elegant physiologic model of nongenomic steroid signaling, as it proceeds completely independent of transcription. We previously demonstrated that androgens are the main physiologic stimulator of oocyte maturation in Xenopus oocytes, and that the adaptor protein paxillin plays a crucial role in mediating this process through a positive feedback loop in which paxillin first enhances Mos protein translation, ensued by Erk2 activation and Erk-dependent phosphorylation of paxillin on serine residues. Phosphoserine-paxillin then further augments Mos protein translation and downstream Erk2 activation, resulting in meiotic progression. We hypothesized that paxillin enhances Mos translation by interacting with embryonic PolyAdenylation Binding Protein (ePABP) on polyadenylated Mos mRNA. Knockdown of ePABP phenocopied paxillin knockdown, with reduced Mos protein expression, Erk2 and Cdk1 activation, as well as oocyte maturation. In both Xenopus oocytes and mammalian cells (HEK-293), paxillin and ePABP constitutively interacted. Testosterone (Xenopus) or EGF (HEK-293) augmented ePABP-paxillin binding, as well as ePABP binding to Mos mRNA (Xenopus), in an Erk-dependent fashion. Thus, ePABP and paxillin work together in an Erk-dependent fashion to enhance Mos protein translation and promote oocyte maturation.
Bag,
Multiple levels of post-transcriptional control of expression of the poly (A)-binding protein.
2010, Pubmed
Bag,
Multiple levels of post-transcriptional control of expression of the poly (A)-binding protein.
2010,
Pubmed
Cosson,
Characterization of the poly(A) binding proteins expressed during oogenesis and early development of Xenopus laevis.
2002,
Pubmed
,
Xenbase
Deng,
The Xenopus laevis isoform of G protein-coupled receptor 3 (GPR3) is a constitutively active cell surface receptor that participates in maintaining meiotic arrest in X. laevis oocytes.
2008,
Pubmed
,
Xenbase
Fisher,
Dissociation of MAP kinase activation and MPF activation in hormone-stimulated maturation of Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Frank-Vaillant,
Two distinct mechanisms control the accumulation of cyclin B1 and Mos in Xenopus oocytes in response to progesterone.
1999,
Pubmed
,
Xenbase
Fraser,
Cellular stress in xenopus kidney cells enhances the phosphorylation of eukaryotic translation initiation factor (eIF)4E and the association of eIF4F with poly(A)-binding protein.
1999,
Pubmed
,
Xenbase
Fraser,
The association of initiation factor 4F with poly(A)-binding protein is enhanced in serum-stimulated Xenopus kidney cells.
1999,
Pubmed
,
Xenbase
Friend,
Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation.
2012,
Pubmed
,
Xenbase
Gallo,
Stimulation of Xenopus oocyte maturation by inhibition of the G-protein alpha S subunit, a component of the plasma membrane and yolk platelet membranes.
1995,
Pubmed
,
Xenbase
Guzeloglu-Kayisli,
Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice.
2012,
Pubmed
,
Xenbase
Guzmán,
A Gbetagamma stimulated adenylyl cyclase is involved in Xenopus laevis oocyte maturation.
2005,
Pubmed
,
Xenbase
Haccard,
Oocyte maturation, Mos and cyclins--a matter of synthesis: two functionally redundant ways to induce meiotic maturation.
2006,
Pubmed
Hammes,
Overlapping nongenomic and genomic actions of thyroid hormone and steroids.
2015,
Pubmed
Howard,
The mitogen-activated protein kinase signaling pathway stimulates mos mRNA cytoplasmic polyadenylation during Xenopus oocyte maturation.
1999,
Pubmed
,
Xenbase
Kahvejian,
Mammalian poly(A)-binding protein is a eukaryotic translation initiation factor, which acts via multiple mechanisms.
2005,
Pubmed
Kim,
RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB.
2007,
Pubmed
,
Xenbase
Lin,
The nuclear experience of CPEB: implications for RNA processing and translational control.
2010,
Pubmed
,
Xenbase
Lowther,
Embryonic Poly(A)-Binding Protein Is Required During Early Stages of Mouse Oocyte Development for Chromatin Organization, Transcriptional Silencing, and Meiotic Competence.
2015,
Pubmed
,
Xenbase
Lutz,
Selective modulation of genomic and nongenomic androgen responses by androgen receptor ligands.
2003,
Pubmed
,
Xenbase
Lutz,
Evidence that androgens are the primary steroids produced by Xenopus laevis ovaries and may signal through the classical androgen receptor to promote oocyte maturation.
2001,
Pubmed
,
Xenbase
Lutz,
G protein beta gamma subunits inhibit nongenomic progesterone-induced signaling and maturation in Xenopus laevis oocytes. Evidence for a release of inhibition mechanism for cell cycle progression.
2000,
Pubmed
,
Xenbase
Mehlmann,
The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes.
2004,
Pubmed
Palmer,
A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1.
1998,
Pubmed
,
Xenbase
Rasar,
Paxillin regulates steroid-triggered meiotic resumption in oocytes by enhancing an all-or-none positive feedback kinase loop.
2006,
Pubmed
,
Xenbase
Ríos-Cardona,
A role for GPRx, a novel GPR3/6/12-related G-protein coupled receptor, in the maintenance of meiotic arrest in Xenopus laevis oocytes.
2008,
Pubmed
,
Xenbase
Salaun,
Poly(A)-binding protein 1 partially relocalizes to the nucleus during herpes simplex virus type 1 infection in an ICP27-independent manner and does not inhibit virus replication.
2010,
Pubmed
Sen,
Androgens regulate ovarian follicular development by increasing follicle stimulating hormone receptor and microRNA-125b expression.
2014,
Pubmed
Sen,
Paxillin regulates androgen- and epidermal growth factor-induced MAPK signaling and cell proliferation in prostate cancer cells.
2010,
Pubmed
,
Xenbase
Sen,
Paxillin mediates extranuclear and intranuclear signaling in prostate cancer proliferation.
2012,
Pubmed
Sheng,
Co-operation of Gsalpha and Gbetagamma in maintaining G2 arrest in Xenopus oocytes.
2005,
Pubmed
,
Xenbase
Smith,
Poly(A)-binding proteins are required for diverse biological processes in metazoans.
2014,
Pubmed
Voeltz,
A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase
Wakiyama,
Interaction of eIF4G with poly(A)-binding protein stimulates translation and is critical for Xenopus oocyte maturation.
2000,
Pubmed
,
Xenbase
Whitaker,
Control of meiotic arrest.
1996,
Pubmed
White,
Specific modulation of nongenomic androgen signaling in the ovary.
2005,
Pubmed
,
Xenbase
Woods,
Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells.
2002,
Pubmed
Woods,
Interaction of paxillin with poly(A)-binding protein 1 and its role in focal adhesion turnover and cell migration.
2005,
Pubmed
Xiong,
A positive-feedback-based bistable 'memory module' that governs a cell fate decision.
2003,
Pubmed
,
Xenbase
Yang,
Embryonic Poly(A)-Binding Protein (EPAB) Is Required for Granulosa Cell EGF Signaling and Cumulus Expansion in Female Mice.
2016,
Pubmed
,
Xenbase