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Development 2008 Apr 01;1357:1283-93. doi: 10.1242/dev.015073.
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Lrig3 regulates neural crest formation in Xenopus by modulating Fgf and Wnt signaling pathways.

Leucine-rich repeats and immunoglobulin-like domains 3 (Lrig3) was identified by microarray analysis among genes that show differential expression during gastrulation in Xenopus laevis. Lrig3 was expressed in the neural plate and neural crest (NC) at neurula stages, and in NC derivatives and other dorsal structures during tailbud stages. A prominent consequence of the morpholino-induced inhibition of Lrig3 expression was impaired NC formation, as revealed by the suppression of marker genes, including Slug, Sox9 and Foxd3. In the NC induction assay involving Chordin plus Wnt3a-injected animal caps, Lrig3 morpholino inhibited expression of Slug, Sox9 and Foxd3, but not of Pax3 and Zic1. In line with this, Lrig3 knockdown prevented NC marker induction by Pax3 and Zic1, suggesting that Lrig3 acts downstream of these two genes in NC formation. Injection of Lrig3 and Wnt3a led to low-level induction of NC markers and enhanced induction of Fgf3, Fgf4 and Fgf8 in animal caps, suggesting a positive role for Lrig3 in Wnt signaling. Lrig3 could attenuate Fgf signaling in animal caps, did interact with Fgf receptor 1 in cultured cells and, according to context, decreased or increased the induction of NC markers by Fgf. We suggest that Lrig3 functions in NC formation in Xenopus by modulating the Wnt and Fgf signaling pathways.

PubMed ID: 18287203
PMC ID: PMC2749967
Article link: Development
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actl6a cer1 chrd dusp6 eea1 egr2 fgf2 fgf3 fgf4 fgf8 fgfr1 foxd3 golga2 gsc inka1 lrig3 mapk1 msx1 myc ncam1 nodal3.1 odc1 pax3 rax sia1 smad7 snai2 sncg sox2 sox9 tbxt tfap2a tfcp2 traf4 twist1 uqcc6 wnt3a wnt8a zic1
Morpholinos: lrig3 MO1

Article Images: [+] show captions
References [+] :
Abu-Elmagd, Frizzled7 mediates canonical Wnt signaling in neural crest induction. 2006, Pubmed, Xenbase