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Nat Commun 2022 Jul 25;131:4306. doi: 10.1038/s41467-022-31949-2.
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Cell landscape of larval and adult Xenopus laevis at single-cell resolution.

Liao Y , Ma L , Guo Q , E W , Fang X , Yang L , Ruan F , Wang J , Zhang P , Sun Z , Chen H , Lin Z , Wang X , Wang X , Sun H , Fang X , Zhou Y , Chen M , Shen W , Guo G , Han X .

The rapid development of high-throughput single-cell RNA sequencing technology offers a good opportunity to dissect cell heterogeneity of animals. A large number of organism-wide single-cell atlases have been constructed for vertebrates such as Homo sapiens, Macaca fascicularis, Mus musculus and Danio rerio. However, an intermediate taxon that links mammals to vertebrates of more ancient origin is still lacking. Here, we construct the first Xenopus cell landscape to date, including larval and adult organs. Common cell lineage-specific transcription factors have been identified in vertebrates, including fish, amphibians and mammals. The comparison of larval and adult erythrocytes identifies stage-specific hemoglobin subtypes, as well as a common type of cluster containing both larval and adult hemoglobin, mainly at NF59. In addition, cell lineages originating from all three layers exhibits both antigen processing and presentation during metamorphosis, indicating a common regulatory mechanism during metamorphosis. Overall, our study provides a large-scale resource for research on Xenopus metamorphosis and adult organs.

PubMed ID: 35879314
Article link: Nat Commun
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: aldh9a1 anxa2 arnt2 atf5 bhlhe40 cebpa chga col1a1 col1a2 crip1 eef1d eif2s3 erg fosl2 foxa1 foxr1 gata1 gfi1b hba1 hbd hes1 hes4 hhex hmgb2 ilf3 jarid2 klf7 krt19 lhx6 lyl1 lypla2 mef2c meox2 mhc1a myog neurod1 nme2 notch1 nr1h5 nr4a2 olig1 olig2 pax6 pepd plek psmb4 psmb5 psmc2 ptf1a rel runx3 s100a11 snai1 sox1 sox10 sox17a sox7 taf13 tal1 tcf4 twist1 twist2 txn ybx3 zan

Article Images: [+] show captions
References [+] :
Aztekin, Secreted inhibitors drive the loss of regeneration competence in Xenopus limbs. 2021, Pubmed, Xenbase