Figure 2. Insect Nudt16 protein is active for decapping RNA. Cap-labeled U8 RNA was incubated in the presence of buffer, Mn2+ and protein (as indicated) for 30 min at 37°C (reaction indicated below). Samples of the reactions were spotted on a TLC, which was developed and then visualized on a phosphorimager. The Xenopus (X29/X-Nudt16) protein, present as a positive control, released m7GDP from the cap-labeled RNA. The two human proteins, which varied by an unrelated amino terminal extension, were both efficient at decapping the U8 RNA. Insect (sharpshooter) Nudt16 protein hydrolyzed the RNA to release the m7GPD cap comigrating with that cleaved by the other orthologs. The human H-Syndesmos protein displays no decapping activity.
Image published in: Taylor MJ and Peculis BA (2008)
© 2008 The Author(s). Creative Commons Attribution-NonCommercial license
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