Fig. 4. Spatial distribution of the XAR1 transcript during neurogenesis detected by whole mount in-situ hybridization. A: At neurula stage 15 the XARl transcript is detected in neuroectoderm. Arrows delineate the neural plate. 6: Stage 21 neurula. XARl RNA is detected in the brain, eyes, and spinal cord. C: Dorsal view of a stage 23 embryo. Strong expression is detected in the CNS on each side of midline. D: Close-up view of XARl RNA in the anterior CNS. The arrows point to the hindbrain-spinal cord junction. E: Stage 35 tadpole. Most of the transcript is localized in the head of the embryo. F: Close-up view of the head of the same embryo. Arrows point to the rhornbomeres of the hindbrain that show strong expression. Expression can also be seen at the midbrain-hindbrain and midbrain-forebrain junction, anterior wall of the forebrain, the eye (outside of this focal plan), and mandibular arches and pharyngeal pouches. G: Expression of XARl in the spinal cord of the same embryo as shown (E). The RNA is expressed in cells aligned in the ventral side of the spinal cord (arrows). Some cells in the midtrunk region of the dorsal fin are also stained. H: A stage 24 neurula stained with XARl sense probe as a control. All embryos presented are albino. In all cases, the dorsal side is up and anterior is to the right. Panel (F) and (G) were taken under differential interference contrast optics, all other panels were bright light optics. sc = spinal cord, nc = notochord.
Image published in: Hemmati-Brivanlou A et al. (1992)
Copyright © 1992. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| acvr2b.L | activin type IIB receptor, actr-iib, actriib, XAR1 | X. laevis | Throughout NF stage 15 | neuroectoderm neural plate pre-chordal neural plate chordal neural plate |
| acvr2b.L | activin type IIB receptor, actr-iib, actriib, XAR1 | X. laevis | Throughout NF stage 21 | neural tube posterior neural tube anterior neural tube optic vesicle |
| acvr2b.L | activin type IIB receptor, actr-iib, actriib, XAR1 | X. laevis | Throughout NF stage 23 | neural tube posterior neural tube anterior neural tube brain forebrain midbrain hindbrain midbrain-hindbrain boundary |
| acvr2b.L | activin type IIB receptor, actr-iib, actriib, XAR1 | X. laevis | Throughout NF stage 35 and 36 | central nervous system brain spinal cord rhombomere rhombomere R2 rhombomere R3 rhombomere R4 rhombomere R5 rhombomere R6 rhombomere R7 rhombomere R8 rhombomere R1 midbrain pharyngeal region diencephalon |
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